The city is part of the Gulfport-Biloxi metropolitan area and the Gulfport-Biloxi-Pascagoula, Mississippi Combined Statistical Area. Pre-Katrina, Biloxi was the third largest city in Mississippi; but with its population losses following that storm, Hattiesburg now has that distinction.
The beachfront of Biloxi lies directly on the Mississippi Sound, with barrier islands scattered off the coast and into the Gulf of Mexico.
Keesler Air Force Base lies within the city and is home to the 81st Training Wing and the 403rd Reserve Wing of the U.S. Air Force.
Biloxi is the smaller of two principal cities of the Gulfport-Biloxi, Mississippi Metropolitan Statistical Area, which is included in the Gulport-Biloxi-Pascagoula Combined Statistical Area.
As of the census of 2000, there were 50,644 people, 19,588 households, and 12,379 families residing in the city. The population density was 1,331.8/sq mi (514.2/km²). There were 22,115 housing units at an average density of 581.6/sq mi (224.5/km²). The racial makeup of the city was 71.43% White, 19.04% African American, 0.49% Native American, 5.11% Asian, 0.11% Pacific Islander, 1.43% from other races, and 2.38% from two or more races. 3.65% of the population are Hispanic or Latino of any race.
There were 19,588 households, out of which 31.4% have children under the age of 18 living with them, 44.6% were married couples living together, 14.0% had a female householder with no husband present, and 36.8% are non-families. 30.1% of all households were made up of individuals and 10.6% had someone living alone who is 65 years of age or older. The average household size was 2.42 and the average family size is 3.02.
In the city the population was spread out with 24.2% under the age of 18, 14.3% from 18 to 24, 30.3% from 25 to 44, 19.2% from 45 to 64, and 12.0% who were 65 years of age or older. The median age was 32 years. For every 100 females there were 101.9 males. For every 100 females age 18 and over, there were 101.6 males.
The median income for a household in the city was $34,106, and the median income for a family was $40,685.
Males had a median income of $28,046 versus $21,267 for females. The per capita income for the city was $17,809. 14.6% of the population and 11.2% of families lived below the poverty line. Out of the total population, 19.6% of those under the age of 18 and 11.7% of those 65 and older were living below the poverty line.
Biloxi is served by Gulfport, Mississippi's Gulfport-Biloxi International Airport.
Biloxi's main highway is U.S. Highway 90 (Beach Boulevard), which runs along the beach and by the casinos. It connects the city to Gulfport and points westward and to Ocean Springs and Pascagoula to the east. The Biloxi-Ocean Springs Bridge across Biloxi Bay was rebuilt following Hurricane Katrina, and was fully reopened in April 2008.
Through the northern sections of the city, Interstate 10 passes through, connecting the city to New Orleans, Houston, Mobile and Jacksonville. Interstate 110 splits off from I-10 at D'Iberville and heads south across the Back Bay of Biloxi to U.S. 90 near Beau Rivage, providing the city with an important hurricane evacuation route.
Other highways serving the area include: